ASPERGILLUS CHEMOTAXONOMY (II) ;FUNGAL AFLATOXIN G (G1 AND G2) PRODUCTION PATTERNS IN 24 DIFFERENT ANAMORPHIC SPECIES OF NORTHERN IRAN ISOLATES LABORATORY CULTURE MEDIA

Mycotoxins are metabolites of fungi capable of having immunotoxic -carcinogenic effects in man and animals. Contamination with mycotoxins is a major problem of food and feeds storage which leads to adversely effects also economic losses influencing the public health and agriculture. While contamination by mycotoxigenic fungi is a major problem, and serum-based methods work on‘‘one substance one assay’’ concept as alternative approach, ELISA was used to identify directly these molds before the toxin production and then cheked by HPLC.Identification and specification of Aspergillus isolates from food and feed or environmental samples have resulted in development of more and more chemical technologies or molecular approaches.. We have started to diverge the attention toward the molecular technology in the clinical, forensic science an d regional agriculture sector in response of toxigen isolates nich by conducting more than 100 isolates obtained from northern provinces of Iran containing 24 distinctive species using ELISA/HPLC immunoassays in a double blinde investigation on whole 10 day old culture substrate resijuesrespectively. According to the findings of the examination of the correlation between aflatoxin values were measured by ELISA to measure the relationship between the Countercurrent out of the all cases (sig:0.00,z:- 621/3) and numerically significant difference exists between them,comparisonly between the average aflatoxin G 1 and aflatoxin G 2 obtained by HPLC method there was not significant differences in the mean values of aflatoxin G1 and G2despite of countercurrent relat ionship which consider these as ‘gold standard’ with reliable specificity and sensitivity regarding to FAO/WHO level definition by μ-ng/kg-g.Identification and typing of Aspergillus from cultures or environmental samples have resulted in development of mor e and more chemical technologies or other molecular approaches.