Single-photon sensitive fast ebCMOS camera system for multiple-target tracking of single fluorophores: application to nano-biophotonics
2011
Nano-biophotonics applications will benefit from new fluorescent microscopy methods based essentially on super-resolution
techniques (beyond the diffraction limit) on large biological structures (membranes) with fast frame
rate (1000 Hz). This trend tends to push the photon detectors to the single-photon counting regime and the
camera acquisition system to real time dynamic multiple-target tracing. The LUSIPHER prototype presented
in this paper aims to give a different approach than those of Electron Multiplied CCD (EMCCD) technology
and try to answer to the stringent demands of the new nano-biophotonics imaging techniques. The electron
bombarded CMOS (ebCMOS) device has the potential to respond to this challenge, thanks to the linear gain of
the accelerating high voltage of the photo-cathode, to the possible ultra fast frame rate of CMOS sensors and to
the single-photon sensitivity. We produced a camera system based on a 640 kPixels ebCMOS with its acquisition
system. The proof of concept for single-photon based tracking for multiple single-emitters is the main result of
this paper.
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