Long-term monitoring of a thermal hydrolysis-anaerobic co-digestion plant treating high-strength organic wastes: Process performance and microbial community dynamics.

Abstract Two parallel anaerobic digesters (8500 m3 capacity each), combined with thermal hydrolysis (TH) pretreatment, co-digesting dewatered sewage sludge, dewatered human feces, and food wastewater were monitored over a 12-month period from start-up to explore the feasibility of field application of the combined process. The waste mixtures before and after pretreatment and the feed and digestate of each digester were taken semimonthly (i.e., 48 samples in total) for analysis of the feed characteristics, process parameters, and digester microbial community structure. The TH pretreatment proved effective in improving the bioavailability of the waste mixture. The solubilization efficiency tended to increase with the particulate organic fraction in the raw mixture. Although fluctuations in the feed characteristics and loading significantly influenced the process and microbial behaviors, the digesters maintained stable performance during the study period. Our results demonstrate that the TH-anaerobic digestion process can achieve an effective and robust treatment of the waste mixture.