The application of microsatellite DNA markers for forensic analysis of koi carp (Cyprinus carpio)
2005
We describe the use of microsatellite
DNA markers, previously developed
for common carp, to investigate
a forensic case involving ornamental koi carp.
Two South African breeders offered koi
strains for sale, which a third breeder
claimed were taken illegally from his ponds.
Screening of four microsatellite markers
provided polymorphic results for koi, demonstrating
their applicability for forensic studies.
Amplification product sizes were comparable
to those published for common carp at three
of the four loci. We observed higher allelic
diversity among koi carp than had been
reported for a panel of inbred common carp.
Coefficients of population differentiation
showed no significant differences between
the populations involved. Consequently, our
analyses could not convincingly prove or
disprove foul play. The latter ambiguity was
most likely the result of sampling constraints
rather than the suitability of the markers.
Our results provide a foundation for future
application of microsatellite markers for
forensic investigation, marker-assisted breeding,
and population diversity analysis in koi.
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