Study on Initial Explants Induction of Litsea cubeba

[Objective] The aim of this study was to explore an explants induction method for L. cubeba. [Method] Different types of L. cubeba stem segments were collected at different time as explants materials for induction and culture. [Result] The results showed that sterilization with 0.1% HgCl2 for 10 min achieved better effects, with an explants contamination rate of 40.0% and a mortality rate of 15.0%; stem segments of the 2nd-5th buds below the terminal bud were better explants materials for initial induction; the best sampling time was January, with an induction rate as high as 81.1%, while the contamination rate was only 11.7%; modified MS + 1.0 mg/L of 6-BA + 0.2 mg/L of NAA was the optimal medium for explants induction of L. cubeba. [Conclusion] Semi-lignified stem segments of L. cubeba collected in January, sterilized with 0.1% HgCl2 for 10 min and cultured in modified MS + 1.0 mg/L of 6-BA + 0.2 mg/L of NAA achieved better induction effects on L. cubeba seedlings.