Pseudomonas aeruginosa Strains Isolated from a Variety of Clinical Sites

The ability of Pseudomonas aeruginosa to adhere to the surface of mucous membranes of the human body or to medical devices is considered the initial step in its colonization and subsequent infection. In the present study, we compared the hemagglutination ability (HA) and adherence ability of wild-type P. aeruginosa strains to different cell lines and to polystyrene. A total of 35 P. aeruginosa strains isolated from clinical specimens were used. HA was quantitatively determined in round-bottomed microtiter plates. The ability of strains to adhere to three different cell lines (Buffalo green monkey kidney, HeLa, and human fetal fibroblasts) was detected by immunofluorescence staining and the adherence of strains to the wells of flat bottom polystyrene tissue culture plates was estimated by the spectrophotometric method.