Highly sensitive detection of 6mA at single-base resolution based on A-C mismatch.
2021
We first demonstrated that 6mA can be selectively recognized based on the selective ligation reaction of DNA ligase toward A-C mismatch and 6mA-C mismatch. This method, when further combined with amplification using RCA, achieved highly sensitive identification of 6mA in dsDNA at single-base resolution.
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