Immunological Response to L-Asparaginase Loaded into Red Blood Cells

For several years, various enzymes loaded into red blood cells have been tried as circulating bioreactors. However, due to the bacterial or fungal origin of these enzymes, the in vivo use of such a bioreactor is questionable as concerned with potential human immunological adverse consequences. L-Asparaginase (L-Asp) is a well known enzyme used as a chemotherapeutic agent, but its use leads to important immunological intolerance reactions after intraveinous administration. Therefore it was a good candidate for such a study. The aim of this paper is to present some results for the immunological consequences following the injection of free or RBC loaded L-Asp in human patients.