Detection of 8-oxoG DNA glycosylase activity and OGG1 transcripts in the rat CNS

Abstract The oxoguanine DNA glycosylase (Ogg1) is a DNA repair enzyme that excises 7,8-dihydro-8-oxoguanine present in DNA damaged by oxidative stress. We have investigated the expression of the OGG1 gene in different regions of the rat CNS. Biochemical studies on brain homogenates of adult rats have shown that Ogg1 nicking activity is present at relatively similar levels in the cerebral cortex, the hypothalamus, the pons and the cerebellum. Following in situ hybridization with radiolabeled OGG1 cDNA or specific antisense oligonucleotides, OGG1 transcripts showed a widespread but heterogeneous distribution pattern among distinct brain regions of adult rats: high levels of this transcript were detected in the CA1–CA3 layers and the gyrus dentate of the hippocampal formation, the piriform cortex, the supraoptic nuclei, the olivary complex as well as in the pyramidal cells of layer V of the cortex and the Purkinje cells of the cerebellum. In peripheral organs such as the lungs, the stomach and the spleen, OGG1 transcript is however expressed in specific subpopulations of cells. Using a semi-quantitative reverse transcription — polymerase chain reaction assay on total mRNA from the frontal cortex, OGG1 mRNA was determined to be expressed with relatively the same levels in 1-day-old and 7-day-old rats as well as in adult rats. These results provide evidence for the widespread expression of the OGG1 gene in developing and adult brains.