An efficient in vitro propagation protocol of Dianthus giganteiformis Borbas subsp. kladovanus (Degen) Soo
2018
Dianthus giganteiformis subsp. kladovanus is an endemic, endangered,
horticulturally appealing perennial plant that can be used for the
revegetation of sand dunes of the Danube region. The appropriate method for
its effective production is micropropagation. For this reason, the
experiments were conducted in order to establish an efficient protocol for
the micropropagation of this subspecies. The sterile culture was initiated
from seeds collected in situ and the germination percentage was high (88%).
According to the results obtained in this study, the multiplication phase
should be performed on an MS medium enriched with 0.1 mg/L BAP and 0.1 mg/L
NAA. The concentration of MS salts significantly influenced rooting, and
higher rooting percentages were obtained on reduced MS media (91.7 - 95%)
than on MS media (73.4 - 76.7%). The addition of NAA slightly increased
rooting percentage (up to 95%). Obtained microplants were successfully
acclimatized (83.3%) in a substrate composed of peat and sand (1: 1; v/v).
Using the protocol presented in this paper, the efficient propagation of D.
giganteiformis spp. kladovanus can be achieved for rapid plant production
aimed at revegetation, biodiversity protection and floricultural production
of this species.
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