REGULATION OF ORGANOGENESIS USING LEAF, INTERNODE AND PETIOLE EXPLANTS IN TYLOPHORA INDICA (BURM. F.) MERR. Research Article

The research study regarding the shoot formation from leaf, internode and petiole explants via organogenesis, subsequent developments and rooting of the in vitro proliferated shoots of Tylophora indica (Burm. f.) Merr. and transplantation of regenerated plants under in vivo condition has been done. Organogenic calli were obtained from leaves of Tylophora indica on MS medium supplemented with BAP (2.0 mg/l) and IBA (0.5 mg/l). Out of different explants tried viz leaf, internode and petiole, leaf gave the optimum organogenic calli with 95% response on MS medium supplemented with BAP (2.0 mg/l) and IBA (0.5 mg/l) in combination. The elongation of developed shoots (12.00±1.50 cm) was obtained on MS medium along with TDZ (0.1 mg/l). Maximal rooting responses (85%) were obtained by the subculture of regenerated shoots on half strength of MS medium supplemented with IBA (0.5 mg/l) after 15 days of incubation. Complete plantlets were hardened, acclimatized and successfully transferred to natural environmental conditions, where they showed 75% survival rate.