THE LIQUIFIED FERMENTATION OF PALM-OIL WASTE WITH RHIZOPUS OLIGOSPORUS TO PRODUCE THE FATTY-ACID OMEGA-3.

Background:The utilization of Rhizopus oligosporus to produce fatty acid omega-3 in liquid substrate has been conducted widely. The content of fat in the palm oil waste in the amount of 5.56/100 gram still has potential to produce fatty acid omega-3. The solid fermentation of tofu waste with R. oligosporus could increase the content of fatty acid up to 34.4% and palm oil waste could increase up to 61.57%. Methods: Sample is collected from the industry of palm-oil. The palm-oil waste is considered as a substrate, and the mold used is R.oligosporus. The waste product is formulated by adding urea and sucrose. In this case, urea is used as the source of N (nitrogen) and sucrose is used as the source of C (carbon). The substrate with nitrogen is called as low-carbon treatment and the substrate with carbon is called as high-carbon treatment. For the controlle we did not add anything. Fermentation is conducted within 7 days on the shaker at room temperature. The product of fermentation was analyzed for the Water, Ash, Fat, and Fatty-Acid Omega-3 contents. Results: This research shows that the water content of the fermentation product decreased and ash content increased. This happened both in the low-carbon treatment and in the high-carbon treatment. The content of fat after fermentation in controlle treatment increased by 6.44%. However, in the low-carbon treatment fermentation, the fat content decreased by 31.14% from the original content. In the high-carbon treatment fermentation, the fat content increased by 31.67%. The content of saturatedfatty acids produced in the high-carbon treatment and low-carbon treatment are as follows: Capric acid (10:0) and lauric acid (12:0) decreased and myristic acid (14:0), palmitic acid (16:0) and stearic acid (18:0) increased, except the myristic-acid in high-carbon treatment decreased . The content of unsaturated-fatty acid in controlle and high-carbon treatment: Oleic acid (18:1), linoleic acid (18:2) and linolenic (18:3) increased. However, all fatty acid in low-carbon treatment decreased, except the linolenic-acid. The conclusion: The fermentation of palm-oil waste with Rhizopus oligosporus mold could increase the content of fat and produce fatty acid omega-3. In addition, the high-carbon substrat could increase the production of unsaturated-fatty acid.