Optimal production enzyme condition of one strain aspergillus Trichoderma sp. TY2 and enzymological characters.

[Objective and Method]The present research was designed to screen cellulase production strain with high activity,to highly use cellulase resources,the strain of fungi named TY2 could decompose cellulose efficiently.It was identified as Trichoderma sp.by morphology identification and 18S rDNA sequence analysis.[Result]The results showed that the optimum cellulase production conditions for TY2 producing cellulase were 1% filter paper,2% wheat bran and 0.5% glucose as carbon sources,0.5% peptone as nitrogen source,40 mL liquid medium in 150 mL flask,inoculation amount 6%,the initial pH 5.0,fermentable temperature 28 ℃,200 r/min of rotation,fermentation time 5 days,the enzyme activity of CMCase and the FPase reached 412.5 U/mL and 100.3 U/mL respectively conditions.A endo-β-glucanase from TY2 strain was separated and purified.The optimal temperature and pH for endo-β-glucanase activity were about 60 ℃ and 4.6,the enzyme activity was stable during the pH 3.0～5.0 and under 50 ℃.It still has the ability of degradation CMC-Na at 80 ℃,Cu2+,Zn2+,K+ and Na+ can activate the activity of endo-β-glucanase,while Hg + inhibited the enzymatic activity.[Conclusion]The strain TY2 has certain applied value in industry.