Optimization on enzymatic reaction conditions of γ-cyclomaltodextrin by CGTase

A strain of Bacillus alkalophilus12-7 which could produce glucanotransferase(CGTase) forming γ-cyclomaltodextrin was screened.Cyclodextrins production technology conditions were studied,and we mainly discussed the influence of enzymatic reaction conditions on γ-cyclodextrins production by glucanotransferase.The results showed that: the enzymatic reaction conditions were pH 8.0,temperature 60 ℃,enzyme amount 500 U/g starch,glycyrrhizic acid concentration 2%,DE 6 ～ 11,yield of CGTase was good,edible Canna Starch and tapioca starch were suitable substrate of CGTase in production of γ-CD,Promozyme in increased the yield of γ-CD was limited.