Lipidomics Strategy for the Identification of Ultra-High Temperature and Reconstituted Milk by UPLC-Q-Exactive Orbitrap Mass Spectrometry

Lipidomic strategies have been applied to many methodologies, such as species differentiation and lipid-associated disease identification. In this study, we first applied a lipidomic strategy coupled with UPLC-Q-Exactive Orbitrap mass spectrometry to investigate the differences between reconstituted milk and ultra-high temperature sterilization (UHT) milk. Fifteen lipids were selected as biomarkers to identify UHT and reconstituted milk by OPLS-DA method. Furthermore, a discriminant model to identify reconstituted milk from UHT milk was established based on these biomarkers. This robust discriminative model provided a strong technical proof to promote the development of the market norms concerning the identification of UHT and reconstituted milk.