Rat adipose tissue secretome differential analysis from different anatomical locations

We performed a 2-DE differential analysis comparing the secretome of fat adipose tissue depots from different anatomical locations. From an average of 1425±SD23.7, 1441±SD14.4 and 1529±SD26.17 spots from visceral, subcutaneous and gonadal adipose secretomes respectively, 204 differences were considered statistically significant when comparing visceral vs subcutaneous vs gonadal secretomes with a pvalue < 0.05, 124 with a p-value <0.01, and 45 with a p<0.001. The most restrictive analysis was chosen for mass spectrometry analysis, thus 45 spots were excised for protein identification. From those, 37 proteins that correspond to 35 ORFs were identified. 74% of the 35 differentially secreted proteins were classified as secreted by non-classical (51%) and classical (23%) pathways.