Determination of stachydrine hydrochloride in Extractum Leonuri Inspissatum by HPLC

OBJECTIVE To establish an HPLC method for the determination of stachydrine hydrochloride in motherwort cream by HPLC.METHODS Prepared test solution with methanol and remove impurities such as sugar,CAPCELL Pak C18 column (5μm,150mm×4.6mm) column for the analysis; methanol-0.05mol·L-1 potassium dihydrogen phosphate solution (67∶33)[1] as the mobile phase; UV detection wavelength of 215nm; column temperature is 30℃; a flow rate of 1.0mL·min-1; By external standard method to calculate the results.RESULTS Stachydrine hydrochloride showed good linearity in the range of 2.05~12.30μg,r=0.9999, the sample average recovery was 98.9%,RSD 0.4%;Repetitive experimental RSD of 0.4%.CONCLUSION The method was simple,accurate,reproducible and can be used as a quality control of Motherwort Cream.