No role of 3435C>T and 2677G>T ABCB1 (MDR1) gene single nucleotide polymorphisms in imatinib treatment response: A case control study on CML patients of Kashmir

Abstract Studies on polymorphisms in ABCB1 (ATP Binding Cassette subfamily B member 1) gene have shown importance in CML treatment. 42 CML patients comprising 18 males (42.86%) and 24 females (57.14%) aged from 7 to 75 years, of which 19 (45.24%) belonged to age group of ≤45 years and the rest 23 (54.76%) were >45 years of age. A case-control analysis of 3435C>T and 2677G>T ABCB1 single nucleotide polymorphisms (SNPs) with respect to imatinib treatment outcome and evaluation of correlation, if any, with molecular (both qualitative & quantitative) and cytogenetic responses of the patients at 01 year was done by Multiplex RT-PCR, PCR-RFLP, quantitative real time PCR and cytogenetics. No association of any statistical significance was noted between either of the two SNPs and the imatinib treatment outcome.