Inhibitory Effects of Chemical Constituents from Actinidia kolomikta on LPS-Induced Inflammatory Responses

Sixteen known compounds, including three lignan glycosides (1–3), seven phenolic glycosides (4–10), three glycosides (11–13), and three flavonoids (14–16), were isolated from the rhizomes of Actinidia kolomikta (Rupr. & Maxim.) Maxim., Actinidiaceae. Their structures were elucidated through extensive spectroscopic analyses and comparison with those reported in the literature. This study is the first confirmation of the presence of the twelve compounds (1–4, 6–13) isolated from the Actinidiaceae family. The isolated compounds were evaluated for their inhibitory effects on NO, TNF-α, and IL-6 production in LPS-induced RAW264.7 cells. Among them, salidroside (9), D-threo-guaiacylglycerol 7-O-β-D-glucopyranoside (10), and epi-afzelechin (15) exhibited the potent anti-inflammatory effect against NO, TNF-α, and IL-6. In addition, compound 10 showed significant inhibitory effects on COX-2 expression at 50 μM, and compound 15 showed significant inhibitory effects on iNOS expression at 50 μM. These results suggest that the anti-inflammatory activity of A. kolomikta might in part occur via the inhibition of the expression of the pro-inflammatory cytokines.