Zwitterionic hydrophilic interaction solid-phase extraction and multi-dimensional mass spectrometry for shotgun lipidomic study of Hypophthalmichthys nobilis.

Abstract Zwitterionic hydrophilic interaction liquid chromatography (ZIC-HILIC) material was used as solid-phase extraction sorbent for purification of phospholipids from Hypophthalmichthys nobilis . The conditions were optimized to be pH 6, flow rate 2.0 mL·min −1 , loading breakthrough volume ⩽ 5 mL, and eluting solvent 5 mL. Afterwards, the extracts were analyzed by multi-dimensional mass spectrometry (MDMS) based shotgun lipidomics; 20 species of phosphatidylcholine (PC), 22 species of phosphatidylethanoamine (PE), 15 species of phosphatidylserine (PS), and 5 species of phosphatidylinositol (PI) were identified, with content 224.1, 124.1, 27.4, and 34.7 μg·g −1 , respectively. The MDMS method was validated in terms of linearity (0.9963–0.9988), LOD (3.7 ng·mL −1 ), LOQ (9.8 ng·mL −1 ), intra-day precision ( H. nobilis .