Characterization of Root-knot Nematodes (Meloidogyne spp.)Associated with Abelmoschus esculentus, Celosia argentea andCorchorus olitorius
2015
Vegetable crops are highly susceptible to a wide range of pests and diseases among which are
the root-knot nematodes (Meloidogyne species). Traditionally, identification of Meloidogyne species
had been based on use of morphological characters such as the perennial pattern. In recent times,
accuracy of nematode identification using only morphological tools has been challenged due to
similarities in morphological characters of some nematode species. The aim of this study was to
identify the root-knot nematodes associated with some vegetable crops cultivated on Covenant
University farm, Ota, South-west, Nigeria using molecular tools and to determine the population
densities of Meloidogyne spp. on the selected vegetable crops. Plant-parasitic nematodes were
extracted from soil and roots of Abelmoschus esculentus, Celosia argentea and Core horus olitorius
cultivated on Covenant University farm. The nematode species were identified and counted under
a compound microscope. The molecular characterization of the Meloidogyne species was done using
single adult female nematodes and eggs which were picked out for DNA extraction and amplified
with specie-specific primers through Polymerase Chain Reaction (PCR) and separated on 0.5%
agarose gel. High population of plant- parasitic nematodes was recorded on the vegetable crops
cultivated on covenant university farm. Also significantly higher population (p<0.005) of
Meloidogyne spp. were found in association with C. argentea and Corchorus olitorius than those
recorded on Abelmoschus esculent us from the vegetable farm. The molecular characterization of
the Meloidogyne species from the farm indicates Meloidogyne incognita as the nematode species
associated with the vegetable crops.
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