Study on Activities of Amylase and Protease of Girella leonina

This paper measures amylase and protease in the four digestive organ of Girella leonina to find how digestive enzyme activity changes given different temperature and pH value.Nine temperature gradient(10~50 ℃) and 14 pH gradient buffer(pH 2.0~9.0) are set up in the experiment conducted in this research using starch-iodine chromogenic method for amylase activity and Folin-phenol reagent method for protease.The results show that the optimal temperature for both amylase of diverticulum and intestine is 35 ℃,their maximal dynamic value of amylase accounts for 46.02 U.g-1 fresh organizations and 42.61 U.g-1 fresh Organization,respectively.For protease,the value are 156.52 U.g-1 fresh organizations and 147.79 U.g-1 fresh Organization,respectively.Stomach enzyme optimum temperature is found to be 40 ℃,and liver amylase optimum temperature requirements is noted for being slightly lower than 30 ℃,which reaches the lowest value of enzyme activity.The optimum pH values for stomach are 6.5 and 3.5,whereas the optimum pH value for diverticulum,intestine and liver tissue turns out to be close to 7.0~8.0.As a result,acid protease is the major protease in stomach,stomach is also the initial decomposition place for digestion.The value of Q10 reflects the influence of temperature on the digestive enzyme activity,so the temperature indicators should be counted as the beginning of feeding.