Differences of the contents of berberine hydrochloride by three preparation methods in Sanhuang Xiexin decoction by HPCE Internal Standard Method.

[Objective] To establish a method to determine the berberine hydrochloride content in Sanhuang Xiexin decoction by HPCE(high performance capillary electrophoresis) Internal Standard Method,and to compare the differences in berberine hydrochloride contents by three different peroration methods.[Method] Diphenyl guanidine was used as the internal standard with a fused silica capillary(50 μm×80 cm,effective length of 71.7 cm) .Other conditions were DAD detector,detection wavelength at 275 nm,25 mmol/L borax-acetonitrile(3∶1,V/V,pH=9.12) solution as the electrophoretic medium,30 kV working voltage,50 kPa sampling pressure for 15 s,5 kPa electrophoretic medium sealing for 5 s,and 15 ℃ column temperature.Thus,the content of berberine hydrochloride in sample was determined.[Result] When the internal standard of diphenyl guanidine was 60.10 μg/ml,berberine hydrochloride showed good linear relationship within the range of 6.40-97.28 μg/ml,with r = 0.999 57,recovery rate=100.17%,and RSD=1.75%.The content of berberine hydrochloride in recipe extracts was significantly higher than in others by this method.[Conclusion] This method was simple,rapid,accurate,good in repeatability,and could be used as the quality detection means for Sanhuang Xiexin decoction.