Integration Analysis of m6A Regulators and m6A-Related Genes in Hepatocellular Carcinoma
2021
Background: N6-Methyladenosine (m6A) RNA methylation of eukaryotic mRNA is involved in the progression
of various tumors. We aimed to investigate m6A-related genes and m6A regulators in
hepatocellular carcinoma (HCC) and their association with prognosis in HCC.
Methods: We downloaded liver cancer sample data from The Cancer Genome Atlas (TCGA) and the
International Cancer Genome Consortium database. A total of 21 m6A regulators and
1258 m6A-related genes were then analyzed by consensus clustering, Spearman’s correlation,
GO, KEGG, LASSO Cox regression, and univariate Cox regression analyses. Finally, we
constructed a risk prognostic model.
Results: We obtained 192 candidate m6A-related genes and 3 m6A regulators, including YTHDF1,
YTHDF2, and YTHDC1. The expression of these genes and regulators differed significantly
in different stages of HCC. Based on Cox regression analysis, 19 of 98 m6A-related
prognostic genes were obtained to construct a risk score model. The 1- and 3-year
area under the curves (AUCs) among HCC patients were greater than 0.7. Finally, based
on analysis of mutation differences between high- and low-risk score groups, we determined
that TP53 had the highest mutation frequency in the high-risk HCC patient group, whereas titin
(
TTN) had the highest mutation frequency in the low-risk HCC patient group.
Conclusion: This study comprehensively analyzed m6A regulators and m6A-related genes through
an integrated bioinformatic analysis, including expression, clustering, protein–protein
interaction, and prognosis, thus providing novel insights into the roles of m6A regulators
and m6A-related genes in HCC.
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