Polymorphism of Isotype 1 β-tubulin Gene of Haemonchus contortus -A Report

Nucleotide sequence analysis of isotype 1 β-tubulin gene of Haemonchus contortus collected from Uttarkashi, Pantnagar and Nainital was carried out to detect single nucleotide polymor phisms (SNP) in the gene associated with anthelmintic resistance. Nucle otide and amino acid sequence analysis of the fragments of the β-tubulin revealed mutation Phenylalanine-200 to Tyrosine (F200Y) in the isolat es from Uttarkashi and Pantnagar while Phenylalanine was detected at codon 200 in the isolates from Nainital. The β-tubulin mutation at Glutamate-198 to Alanine (E198A) and Phenylalanine-167 to Tyrosine (F167Y) was not detected in any of the isolates. This shows that isolates from Uttarkashi and Pantnagar were resistant containing mutation F200Y. The isola te from Nainital was susceptible with none of the SNP at codon 200, 198 or 167. For effective worm control, it is important to know the status of anthelmintic efficacy in a particular agro-climatic zone. This will not only delay spread and rise of benzimidazole resistance in Uttarakhand but also th e life span of usage of these benzimidazoles could be extended.