Synergistic effects of arsenic trioxide and proteasome inhibitor bortezomib on apoptosis induction in Raji cell line

The aim of this study was to explore the synergistic effect of arsenic trioxide and bortezomib on apoptosis of Raji cell line.The cells were treated with arsenic trioxide,bortezomib,low-dose arsenic trioxide combined with bortezomib,respectively.The cell viability and proliferative curve were estimated by trypan blue dye exclusion.The cell apoptosis and cell cycle status were analyzed by flow cytometry.The apoptosis related elements such as caspase-3,BCL-2,BAX,JNK2 and IκB-α,were measured with Western blot.The results showed that compared with cells treated with mentioned above drugs alone,the proliferative potential of cells in combination group was significantly inhibited(p0.01),and apoptosis rate markedly increased(p=0.001),while obvious cell cycle arrest was not observed.On the protein level,the expression of Caspase-3,BAX and IκB-α increased,while the expression of BCL-2,and JNK2 decreased.It is concluded that low-dose arsenic trioxide combined with bortezomib synergistically induced apoptosis in Raji cell line which may be mediated by inhibiting NK-κB and JNK2 signaling.