Білковий профіль крові свиней за згодовування їм ГМ-сої

CHANGING IN PROTEIN PROFILE OF PIGS BLOOD DURING FEEDING WITH GM SOYBEANS S.G. Zinoviev, S.O. Semenov, A.A. Bindyug, D.O. Bindyug Currently, the most widely use genetic engineering found in creating new varieties of crops those have missing features in the existing traditional plants. Resistant to herbicides soybeans has become the most common in agricultural production, more than 60% of soybeans are modified and resistant to pests; certain modified varieties of cotton and corn 28% and 14% are grown as well. Despite a long period of use of GM feed in animal breeding gene modified products are known to influence sometimes harmfully on living organisms. However, there are no scientific data confirming the negative impact of genetically modified organisms (GMOs) on health and physiological state of laboratory animals. So, the final answer on the safety of GM feed for animals worldwide has not been granted yet, and most studies try to study it influence. Therefore, research on the effects of use of modified feed in livestock, particularly influence on pig health and other animals is very relevant The purpose of research - the study of changes in blood protein profile for using in swine diets of feeding with genetically modified soybeans. Material and methods research . Research conducted in accordance with international principles of the European Convention for the Protection of vertebrate animals used for experiments on them and other scientific purposes. Scientific and economic research conducted on pigs Poltava meat breed, kept in conditions of state enterprise "experimental basis "Nadiya" Institute pig and APV. From the group of pig offspring 24 animals were taken, was selected chairman of clinically healthy pigs live weight analogues, and formed two experimental groups, which included 8 female pigs and 4 male. The composition of diet feeding from the control group of animals was included in equal amounts by weight (10%), full-fat soy extrusions without genetic modification, and research - gm full-fat soy extrusions (RR, GTS 40.3.2). After reaching 4 and 8-month age, fasting from ear edge vein blood was collected for analysis. Biochemical blood parameters were determined using commercial kits Ukrainian company "Filisit Diagnosis": total protein – by biuret reaction (g / l), protein fractions - turbidimetric method. Statistical analysis of the data was performed using the programs Microsoft Exel 2013 and Statistica 8.0. Results and discussion . According to the data, the blood protein profile pigs those received one of the feed ingredient which has been genetically modified soybeans, slightly changed comparing to the control animals. The level of total protein in the blood of young pigs 4-month of age in both groups was virtually identical. However, in 8-month increased its concentration in the control and experimental groups according to 99,11 ± 11,235 g / l and 89,29 ± 3,858 g / l. Despite the fact that the initial research period (4 mo.) Amount of albumin in the blood of pigs experimental group was significantly lower at 22.98% (p = 0.000056) comparing to controls, in 8-month to content blood pigs in both groups significantly (p = 0.000001, p = 0.0019) increased, did not differ among themselves and were respectively 47.40 and 48.95 g / l. The concentration of α1 globulin in the blood of pigs 4 and 8-month in various groups in the presence of GMO diets did not differ significantly, but slightly decreased with age in both groups of animals. In 8-month group concentration in the control and the experimental groups was 5.60 and 4.95 g / l, respectively, less than the initial period of young pigs at 24.84 and 24.43%. The concentration of α2 globulin in the blood of experimental animals varied depending on the age and composition of the diet, with the years it dropped to 48.42% in the control and 63.08% (p = 0.0005) in the experimental. Essential but unreliable, due to the high variability in 8-month group of age, decreased concentration specified globulin in pigs in the control group at 8-month group of age and amounted to 15,80 g / l. In 8-month group pigs fed GM soy content α2 globulin was lower at 21.54% (p = 0.001) compared with controls. This change their content, in our view, can indirectly indicate a decrease in estrogen levels in the blood of pigs experimental group that in the future could adversely affect their reproductive ability. Globulin fraction β has also undergone significant decrease in the concentration of age respectively 37.20% in the control group (p = 0.002) and 32.29% (P = 0.01) in the experimental group, but the factor feeding the specified index is not affected. The content of γ globulin in the blood of experimental animals in both groups of 4 to 8-month groupof age also decreased respectively by 28.67% and 44.77% (p = 0.00001). Given that 4 monthly experimental group of animals, their concentration in the blood was at 39.90% (p = 0.006) higher comparing to the control animals, reducing their content in 8-month group was much more significant. Nevertheless, in the blood 8-monthl group number of experimental animals γ globulin was significantly (p = 0.0005) higher comparing with control group at 24.33% to 18.65 g / l. Age-related changes in the concentration of albumin (growth) and globulin (decrease) affected the albumin-globulin ratio in the blood of pigs control and research groups: it grew by 128.57% (p = 0.00003) and 184.38% (p = 0.004). Key words: pigs, soybean, GMO, blood, metabolism, total protein, protein fractions