Examining submerged and solid-state cultivation course of hydrolytic enzymes production from wheat chaff

Agricultural waste represents an interesting raw material for biotechnological processes nowadays, due to its low price, favorable composition and availability. Wheat chaff, as a lignocellulosic by-product of wheat processing could be a suitable renewable source for producing hydrolytic enzymes for second generation ethanol production technologies. The aim of this work was to investigate the course of Trichoderma reesei cultivation for hydrolytic enzymes production by submerged and solid-state techniques on medium based on wheat chaff. Results show that the monitored values (hydrolytic enzymes activities, total protein content and reducing sugars content) vary significantly with cultivation time, thus there is a need for further optimization of this process parameter.