Scavenging effects of porphyrin-fluorescein hybrid on scavenging of reactive oxygen species and inhibition of oxidative damages in vitro

Objective Study the scavenging effects of porphyrin-fluorescein hybrid on scavenging of reactive oxygen species and inhibition of oxidative damages in vitro.Methods The scavenging effects of porphyrin-fluorescein hybrid on reactive oxygen species·OH and O2-· generated by Fenton reaction and riboflavin photosensitization were investigated by the means of spectrophotometry.And the inhibition effects of porphyrin-fluorescein hybrid on lipid peroxidation in the presence of lecithin and oxidation damage of DNA chain induced by hydroxyl radical were observed by spectrophotometry with thiobarbituric acid.Results The results showed that porphyrin-fluorescein hybrid could scavenge reactive oxygen species efficiently.It could also significantly inhibit lipid peroxidation in the presence of lecithin and oxidation damage of DNA by hydroxyl radical.Conclusion The porphyrin-fluorescein hybrid was an effective antioxidant in vitro.