A comparison of methods for the measurement of 8-isoPGF2α: a marker of oxidative stress

BackgroundOxidative stress describes the cellular damage caused by excess reactive oxygen species not adequately inactivated by antioxidants. Oxidative stress has been implicated in playing a role in many disorders. Lipid peroxidation end-products are employed as markers of oxidative stress, of which the isoprostane, 8-iso-PGF2α, is widely used. 8-iso-PGF2α is measured in plasma or urine by gas chromatography–mass spectrometry (GC/MS), liquid chromatography–mass spectrometry (LC/MS), tandem-mass spectrometry or enzyme-linked immunosorbent assay (ELISA). However, discrepancies between the specificity of these methods means correlation is poor.MethodsA tandem-mass spectrometric (LC/MS/MS) method, using immunoaffinity purification, for urinary 8-iso-PGF2α was developed and compared with two commercial ELISAs (A – Cayman Chemicals, B – Oxford Biomedical Research) in urine samples (n = 156).ResultsAn LC/MS/MS method coupled to immunoaffinity purification was developed with satisfactory performance and comparis...