In vitro hepatoprotective activity of Indigofera barberi gamble against d-galactosamine induced toxicity

The present study is aimed to detect the In vitro hepatoprotective nature of isolated compound from Indigofera barberi Gamble belonging to the family Fabaceae. In vitro hepatoprotective potential of isolated compound from indigofera barberi was evaluated using HepG2 cells. Based on the cytotoxicity assay, isolated compound (10, 100, 1000 µg/ml) was assessed for hepatoprotective potential against D-Galactosamine induced toxicity in HepG2 cell line by monitoring cell viability, aspartate amino transferase (AST), alanine amino transaminase (ALT), Alkaline Phosphatase (ALP), Gamma Glutamyl Transpeptidase (gGTP), lipid peroxidation (LPO) and glutathione level (GSH). The results indicated that D-Galactosamine treatment caused a significant decrease in cell viability. HepG2 cells showed significant dose depended increase in percentage viability at the dose 10, 100 and 1000 µg/ml of isolated compound compared to D-Galactosamine exposed HepG2 cells. The percentage viability of HepG2 cells after incubation with D-Galactosamine was 18.75± 0.97%, whereas the isolated compound IB-4 (1000 µg/ml) restored the viability to 79.23± 1.47% and aspartate aminotransferase levels to 160.4 ±1.35 U/L. Isolated compound from Indigofera barberi significantly prevented the increase in LPO and GSH level which was brought to near normal. The effect of isolated compound was comparable with that of standard drug Silymarin. The D-Galactosamine induced changes in the HepG2 cells were significantly ameliorated by treatment of isolated compound from Indigofera barberi and also strongly revealed that compound isolated from Indigofera barberi having good hepatoprotective potential.