Genetic Variation Analysis of 3D Gene and Molecular Detection of Porcine Kobuvirus in 2013-2014

Objective] This study aimed to investigate the prevalence and variation of porcine kobuvirus (PKV) in suckling piglets in China. [Method] ln 2013-2014, 224 feces samples from suckling piglets with diarrhea in 27 pig farms of five provinces in China were col ected to detect 3D genes of PKV with RT-PCR method; the se-quences and genetic variation of 29 PKV 3D genes were analyzed. [Result] Total positive rate of PKV in feces samples from suckling piglets with diarrhea was 65.18% (146/224); total positive rate of PKV in pig farms was 85.2% (23/27); nu-cleotide sequences and the deduced amino acid sequences of 29 PKV 3D genes shared 87.0%-100% and 92.7%-100% homologies with six PKV-related 3D se-quences, respectively. [Conclusion] PKV infection is prevalent in suckling piglets in China; PKV 3D genes exhibit high diversity.