High expression of USP18 is associated with the growth of colorectal carcinoma.
2021
Aim. To investigate whether USP18 can be
used as a predictive marker for the diagnosis and
development of colorectal cancer.
Methods. The Gene Expression Omnibus (GEO)
Dataset and the Cancer Genome Atlas (TCGA) database
were used to select differential proteins for the ubiquitinspecific peptidases (USPs). The extensive target
prediction and network analysis methods were used to
assess the association with the USP18 interacting
proteins, as well as the statistical correlation between
USP18 and the clinical pathology parameters. The
effects of USP18 on the proliferation of colorectal
cancer were examined using CCK8. The effects of
USP18 on the migration of colorectal cancer were
examined using wound healing assays. Immunohistochemistry (IHC) was performed on the tissue microarray.
Results. The results showed that the expression of
USP18 was related to age (P=0.014). The positive rates
of the USP18 protein in T1, T2, T3, and T4 were 0.00%,
22.92%, 78.38%, and 95.35%, respectively (P<0.00).
The positive rates of the USP18 protein in I, II, III, and
IV were 47.43%, 83.12%, 66.67%, and 100.00%,
respectively (P<0.00). The Western blot assay showed
that the expression of USP18 in colorectal cancer tissues
was significantly higher than that in matched
paracancerous tissues (P<0.05). The CCK8 experiments
suggested that USP18 promoted the migration of CRC
cells. Wound healing assays suggested that USP18
promoted the proliferation of CRC cells.
Conclusion. This study showed that USP18 can
promote the proliferation of colorectal cancer cells and
might be a potential biomarker for the diagnosis of CRC.
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