Effects of PLGA Degradation on Angiogenesis in vitro

Objective: To investigate the influences of degradable PLGA scaffold on proliferation,migration and the formation of tube-like structure(TLS formation) of vascular endothelial cells.Methods: PLGA scaffold were immersed in PBS fluid to stimulate the degradation for 1,2,and 4 weeks.HUVEC(Human Umbilical Vein Endothelial Cells) was cultured with degradation fluid.Cell proliferation,migration and TLS formation were detected by Brdu ELISA,Transwell chamber and tube formation.Results: The PLGA degradation fluid showed no effects on the migration,TLS formation of HUVEC,but the proliferation was increased in 1 week.With the prolonged degradation time,the migration and TLS formation were significantly decreased in 2 weeks,and the proliferation,migration and TLS formation were reduced remarkably in 4 weeks.Conclusions: At the beginning of degradation,PLGA could improve the proliferation function.The concentration of acid products increased with the increase of degradation time,and cell behaviors were retrained,thus inhibiting the angiogenesis of endothelial cells.