Hepatoprotective effect of Hippophae rhamnoides seed oil on the CCl_4-reduced injuries

Objective To study the protective effect of hippophae rhamnoides seed oil on carbon tetrachloride(CCl4)-induced hepatic injuries and to explore its effective dose.Methods Seventy-two male SD rats were randomly divided into 6 groups:negative control,solvent control,CCl4 model,and low-(0.167 g/kg bw),mid-(0.333 g/kg bw)and high-hippophae rhamnoides seed oil(0.500 g/kg bw)treatment group.Rats in hippophae rhamnoides seed oil group were given different dosage of hippophae rhamnoides seed oil by gavage once a day,while rats in negative control group were given distilled water,and rats in solvent control and CCl4 model group were given peanut oil respectively with the same manner.After 45 days,rats in CCl4 model group and hippophae rhamnoides seed oil groups were fasted for 16 h and then given one-time CCl4(200 mg/kg bw) by gavage.Rats were euthanized twenty-four hours later after the treatment.The serum ALT and AST of rats were tested with an automatic bioche-mical analyzer for all the groups.The liver weight,coefficient and histopathology examination were evaluated by regular methods.Results The serum concentrations of ALT and AST were significantly lower in rats treated with the low-,mid-and high-dosage of hippophae rhamnoides seed oil than those in rats of CCl4 model group(2 419±1 335,2 832±1 074,2 424±955,4 396±3 026,4 579±990,5 106±1 479,4 503±782,21 861±14 291 nmol·s-1/L)(P0.05).The CCl4-induced hepatocyte injuries were alleviated significantly in the group of rats treated with 0.500 g/kg bw hippophae rhamnoides seed oil(136.0±47.0 vs 288.0±70.0)(P0.05).Conclusion Hippophae rhamnoides seed oil protected rats from CCl4-induced acute hepatic injuries effectively with the dose of 0.5 g/kg bw.