[Simultaneous determination of ephedrine hydrochloride, D-pseudoephedrine and amygdalin in xiao'er pingchuan qutan granule by HPLC].

Objective: To establish an HPLC method for the determination of ephedrine hydrochloride,D-pseudo-ephedrine and amygdalin in Xiao′er Pingchuan Qutan granule.Method: Pheny ether chromatographic column(4.6 mm×250 mm,5 μm) was adopted,with acetonitrile-0.1% phosphoric acid(containing 0.1% three ethylamine)(3∶97) as the mobile phase.The UV detection wavelength was at 210 nm,with the flow rate of 1 mL·min-1,and column temperature was at 35 ℃.Result: The linearity of ephedrine hydrochloride,D-pseudo-ephedrine and amygdalin ranged between 0.078 60-3.144 μg(r=1.000 0),0.103 4-2.068 μg(r=0.999 7) and 0.430 5-3.157 μg(r=0.999 8),respectively.Their average recoveries were 98.46%(RSD 1.1%),103.0%(RSD 1.5%) and 97.15%(RSD 2.1%),respectively.Conclusion: The method is simple,stable and reliable that it can be used to determine the content of ephedrine hydrochloride,D-pseudo-ephedrine and amygdalin in Xiao′er Pingchuan Qutan granule.