A SIMPLE, RAPID, EFFICIENT AND LOW COST METHOD FOR MINIPREP DNA FROM DIFFERENT SOURCES

DNA extraction and purification are routine processes in mostplant genetic transformation laboratories. Although there are differentcommercial kits that allow accurate DNA purification, the total cost ofbuying multiple sets of these kits can be spectacular. We use spincolumn and laboratory solutions to develop a simple method of DNApurification that can meet different research needs. This method is usedto extract DNA from the leaves of Brassica and genetically modifiedplants and also bacteria; extract plasmid DNA from E. coli orAgrobacterium tumefaciens; purify the DNA fragments of PCRproducts and the resulting fragments of digestion with restrictionendonuclease. DNA concentration of optical density (OD) value wascalculated at 260 nm wavelength and OD260 / OD280 ratios were usedto determine DNA quality. The quantity and quality of DNA obtainedby this method was similar to that of isolated DNA using commercialKits. In comparison, it has been shown that this method allowsobtaining DNA from different sources with similar quantity and purityand low costs.