Isolation of CD3−, CD4−, CD8−, IL-2R+ thymocyte precursors by panning

Abstract We present a panning method for isolation of thymocytes that are CD3 − , CD4 − , CD8 − and IL-2R + . These cells have been isolated by positive selection on dishes coated with treatment with biotinylated 145-2C11, GK1.5, and 53-6.7 antibodies and negative selection on avidin coated dishes.