Synthesis of amidine and bis amidine derivatives and their evaluation for anti-inflammatory and anticancer activity.

Abstract 2-Cyanopyridine ( 1a ), 4-cyanopyridine ( 1b ), 2-cyanopyrazine ( 1c ) on condensation with mono amines ( 2a – c ) and diamines ( 4a – c ) in the presence of sodium methoxide as catalyst gave amidine derivatives ( 3a – i ) and bis amidine derivatives ( 5a – i ) in good yields. All these compounds were fully characterized by spectroscopic means and elemental analysis. On screening for anti-inflammatory activity and for in vitro anticancer activity compounds 5c and 5d exhibited good anti-inflammatory activity whereas compounds 5d breast (T47D), 5h , 5i lung (NCI H-522), 5i colon (HCT-15), 3c , 3h , 5i ovary (PA-1) and 3c , 5b , 5h liver (HepG2) exhibited good anticancer activity.