Fungitoxicity of natural heterocycle glucoside vicine obtained from Vicia faba L. against selected microscopic filamentous fungi

Vicine and convicine were isolated from mature seeds of Vicia faba L. These compounds were separated from the relevant fractions by HPLC and identified by IR, MS and NMR. Obtained vicine was used for all bioassays. Vicine in concentration of 25 µg in 1 ml of medium had significant toxic effect. The concentration of 5 µg of vicine in 1 ml of medium had significant fungistatic effect for fungi Fusarium culmorum and Alternaria alternata. The concentration of 500 ng of vicine in 1 ml of medium had significant fungistatic effect for fungi Cladosporium herbarum and Botrytis cinerea. The effective concentration of vicine in our test was significantly lower than is the concentration of vicine in seeds and pods of V. faba. The toxicity of vicine comparing with the toxicity of insecticide Actellic was higher by one order. Also, the fungicide Impact had a lower toxicity than vicine. Fungitoxic and fungistatic effect of vicine was comparable with some other tested fungicides and with the plant saponin digitonin. Our results confirmed that vicine is important natural fungicide.