PARALLEL QUANTITATIVE ESTIMATION OF GALLIC ACID IN AQUEOUS EXTRACT OF EMBLICA OFFICINALIS AND POLY-HERBAL DOSAGE FORM (CAPSULE) BY HPTLC TO AUTHENTICATE THE RATIO OF THIS INGREDIENT DELIVERED TO THE FINAL FORMULATION

The present study is carried out to examine and optimize the method to quantify the presence of Gallic acid, using HPTLC, in ingredient (aqueous extract of Emblica officinalis) as well as in a coded formulation containing extracts of four different herbs in equal quantity developed pharmaceutically in dosage form of capsules. In herbal industry HPTLC has made deeper inroads as method of choice for analysis and documentation of herbal drugs. In the present study the authors have taken utmost care to detect the ratio of marker (Gallic acid) in the aqueous extract of Emblica officinalis as well as in final compound formulation to correspond this ingredient’s ratio in the final dosage form. The Chromatographic analysis was performed on CAMAG HPTLC System using Linomat IV applicator. Pre-coated TLC plates (Merck-code 1.05554.0007) Silica gel 60 F254 were used for analysis. The mobile phase comprised of Toluene : Ethyl acetate: Formic acid::2:5:1.5 v/v and 254nm was detection wavelength. The regression equation showed good linearity in the range of 100-500μg/mL for Gallic acid (R 2 > 0.998) between the peak areas of each marker and concentration. The solvent front was run upto 9 cms. The gallic acid concentration in aqueous extract as well as in a coded formulation was found to be 7.83% and 1.59% respectively.