[Establishment and clinical application of TapMan real-time RT-PCR method for detection of HHV-6].

Objective To establish a rapid,sensitive and specific real-time PCR method for detection of Human Herpesvirus-6 (HHV-6).Methods According to thc rcfcrcncc,a pair of primcrs and a probe were designed located in U65-66 gene and to set up the standards.We established a real-time RT-PCR method for detection of HHV-6,and to verify the specificity,sensitivity,reproducibility.Results The correlation coefficient was 0.999,E =97.9％,the coefficient of variation values of Ct were 0.61％ and 3.13％ in real-time PCR assay for inter and intra assay,respectively.The results of all viruses were negative except of HHV-6 for the assay.The quantitative detection limit of the assay was 3 × 10(0) copies/μl.Conclusion The real-time PCR assay is highly specific,sensitive and reproducible,which can be used to quatitative detecting clinical samples. Key words: Herpesvirus-6, human; Polymerase chain reaction