Mucilaginibacter herbaticus sp. nov., isolated from the rhizosphere of the medicinal plant Angelica sinensis.

A strictly aerobic, Gram-staining-negative, non-motile and rod-shaped bacterial strain, DR-9T, was isolated from rhizosphere soil of the medicinal herb Angelica sinensis. Strain DR-9T grew at 20–40 °C, at pH 4.0–9.0 and in the presence of 0–1 % (w/v) NaCl. The major fatty acids were iso-C15 : 0 and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c and/or iso-C15 : 0 2-OH), MK-7 was the major isoprenoid quinone, and phosphatidylethanolamine and an unidentified aminophospholipid were the major polar lipids. A phylogenetic tree based on 16S rRNA gene sequences showed that strain DR-9T formed a lineage within the genus Mucilaginibacter and was closely related to Mucilaginibacter polysacchareus DRP28T (96.1 % sequence similarity), Mucilaginibacter myungsuensis HMD1056T (95.9 % sequence similarity), Mucilaginibacter ximonensis XM-003T (95.8 %) and Mucilaginibacter boryungensis BDR-9T (95.1 %). The status of strain DR-9T as a representative of a separate species was confirmed by DNA hybridization, with 38.6, 36.3 and 29.9 % DNA–DNA relatedness with M. polysacchareus DRP28T, M. ximonensis XM-003T and M. boryungensis BDR-9T, respectively. The genomic DNA G+C content of strain DR-9T was 49.8 %. These data suggest that strain DR-9T should be considered as a representative of a novel species of the genus Mucilaginibacter , for which the name Mucilaginibacter herbaticus sp. nov. is proposed. The type strain is DR-9T ( = KACC 16469T = NBRC 108839T).