Preparation of an antigen and development of a monoclonal antibody against mono-butyl phthalate (MBP)

Abstract Butyl benzyl phthalate (BBP) is widely used as a plasticizer in carrier of pesticide, food packing and many daily products. It could be hydrolysed to mono-butyl phthalate (MBP) inside the body of organisms, which is highly toxic to humans and other organisms, affecting the reproductive and developmental function. Immune spleen cells were collected from mice immunised intraperitoneally (i.p.) and multiple subcutaneously (s.c.). A hybridoma cell line named 2F7 was obtained via colony hybridisation of immune spleen cells and myeloma cells SP2/0. The mAb against MBP secreted by 2F7 was an isotype of IgG1, and showed 1.93×108 of an affinity and the CR values of the mAb with other analogues of MBP were not more than 5%, except for BBP (11.5%). The high affinity and IgG characteristics of the mAb against MBP could be used for MBP detection in the future.