Negative Pressure Difference Control in Airtight High-level Bio-safety Laboratory

A new concept of air leakage change rate was put forward,which could be used as the guideline for estimating whether the air volume valve could be applicable to the negative pressure difference control in bio-safety laboratory;and a strategy of introducing man-made air leakage for pressure difference control in airtight bio-safety laboratory was proposed.Comparison experiments were conducted to test the pressure fluctuation at the state of laboratory startup,small disturbance,door closing in the laboratory on natural air leakage condition and introduced man-made air leakage condition respectively,and the results indicate that the fluctuating range of pressure difference becomes small and goes to stable more rapidly on introduced man-made air leakage condition.It shows that the method of introducing man-made air leakage for pressure difference control is applicable to airtight bio-safety laboratory.