Enhanced Multiplexing Capabilities of Agilent Technologies SureSelect™ Target Enrichment System for Next-Generation Sequencing

Demand has never been greater for revolutionary technologies that deliver fast, inexpensive and accurate genome information. Massively parallel sequencing technologies have enabled scientists to discover rare mutations, structural variants, and novel transcripts at an unprecedented rate. To meet the demand for fast, inexpensive and accurate genome analysis method, Agilent Technologies has developed the SureSelect platform, an in-solution hybrid selection technology for systematic re-sequencing of user specific genomic regions. With the implementation of this new technology there is a balancing act of cost, quality and quantity and it is easier for scientists to sequence entire genomes from large sample cohorts. The inexpensive production of large volumes of user specific sequence data is SureSelect's primary advantage over conventional methods. To further reduce costs and take advantage of the increasing capacity of next-generation sequencers, such as the HiSeq2000 and the SOLiD4/4hq, we highlight the ability to multiplex DNA samples in a single sequencing lane/slide while maintaining the coverage necessary to confidently make SNP calls. SureSlelect multiplexing kits have an automation-friendly, easy to use protocol where gDNA libraries are uniquely “tagged” and then combined via mass balance on one flow cell lane/slide. We show high performance across both Illumina and SOLiD multiplexing platforms, as measured by capture efficiency, uniformity and reproducibility. The multiplexing capabilities SureSelect make it a cost effective way to study human and mouse exome, or any user defined region of interest. When multiplexing HapMap samples, >98% concordance between SureSelect re-sequencing results and previously determined genotype is observed. Lastly, we introduce the SureSelect XT kit for preparation of samples for multiplex sequencing using the Illumina GAII or HiSeq. The SureSelect Multiplexing kit provides the ability to combine targeted enrichment with multiplexing, thus maximizing the number of samples that can be sequenced at one time, providing optimum time and cost savings without sacrificing performance.