168-P: ASSOCIATION OF HLA AND MICA ALLELES WITH LEPROSY IN FAMILIES FROM AN ENDEMIC AREA OF THE NORTH OF BRAZIL

Aim The objective of this study was to evaluate the influence of HLA and MICA alleles in the development of leprosy and its clinical forms in a population with leprosy from the region of Santo Antonio do Prata – PA, Brazil. Methods Fifty-one families were genotyped for HLA and MICA genes by PCR-SSOP, LuminexO methodology (One Lambda) and Dynal AutoRELI TM 48 Instrument (Invitrogen) Dynal-RELI TM SSO HLA Typing. Statistical Analysis was based on the Transmission Disequilibrium Test (TDT) software FBAT 2.0.4. Results The alleles HLA-A ∗ 03/11 (P=0.041), HLA-C ∗ 04 (P=0.025), HLA-DRB1 ∗ 16 (P=0.048), DQB1 ∗ 05 (P=0.004) and MICA ∗ 002 (P = 0.003) showed significant positive association with the occurrence of leprosy in this population, while the alleles HLA-C ∗ 12 (P = 0.095), DRB1 ∗ 15/16 (P = 0.055), and MICA ∗ 008 (P = 0.075) showed a tendency to positive association. Conclusions This study suggests that the alleles HLA-A ∗ 03/ ∗ 11 , HLA-C ∗ 04, HLA-DRB1 ∗ 16 , DQB1 ∗ 05 and MICA ∗ 002 may be associated with susceptibility to leprosy in this population and HLA-C ∗ 12, DRB1 ∗ 15/16 and MICA ∗ 008 should be further investigated.