Effect of cyclopamine on Nurr1 gene expression in LN229 cells

Objective To investigate the effect of cyclopamine on the expression of Parkinson-related gene Nurr1 in malignant glioma cell line LN229.Methods Upon LPS stimulation,real time PCR was used to detect contents of SHH relational gene PTCH,Gli1and Nurr1 mRNA in LN229 cell.Western blotting was used to detect the impact of LPS on Nurr1 protein expression.Furthermore,the contents of both of Nurr1 and the downstream Inflammation factor IL-1β were detected after blocking SHH Signaling pathway with cyclopamine.Results Upon the LPS stimulation,the relational gene mRNA and Nurr1mRNA in LN229 cell elevated.LPS significantly elevated the expression of nurr1 protein.Contrast with the control group,it was significantly promoted on the expression of Nurr1 gene and the downstream Inflammation factor IL-1β at the level of transcription and translation after cyclopamine blocking.Conclusions The effects of cyclopamine on the Nurr1 gene expression in LN229 cell may be related to the activation of glial cells during the pathogenesis process of Parkinson's disease.