Effects of fosinopril on proliferation and secretion of extracellular matrix of rat glomerular mesangial cell

Objective To observe the effects of fosinopril (FOS) on proliferation and secretion of extraeellular matrix of rat glomerular mesangial cell induced by LPS.Methods In vitro culture method for glomerular mesangial cells (GMC) of rat was established and passages 3-10 of the cells were used in the experiment after identification.The experiment included the following 5 groups:control group (Ctrl),LPS group (LPS),high,medium and low dose FOS groups (FOS1,FOS2 and FOS3 groups,respectively). GMC proliferation was detected by methyl thiazolyl tetrazolium (MTT) incorporation method at 24 and 48 h; the changes of laminin (LN),fibroneetin(FN) and Col Ⅳ protein secretion was detected by the enzyme- linked immunosorbent assay (ELISA).The changes of LNβ_2 mRNA expression was detected by semi- quantitative real-time RT-PCR.Results (1) LPS could induce the mesangial cell proliferation,FOS inhibited this effect of proliferation induced by LPS.(2) Mesangial cells could secrete some extracellular matrix (ECM) protein in normal culture medium,mesangial cell secreted ECM protein was significandy higher in LPS group than that in Ctrl group (P0.01),but significantly lower in all FOS groups than that in LPS group (P0.01).(3) Mesangial cell could express LNβ_2 mRNA in normal culture medium,LN[32 mRNA expression was significantly higher in LPS group than that in Ctrl group at all time points,but was significantly lower in FOS group than that in LPS group.Conclusions LPS could induce increased secretion of the ECM,including LN,FN,Col IV;FOS could inhibit the secretion of ECM in GMC in a dose- dependent manner at mRNA and protein levels.