Study on passage stability of HEK293 cell line by short tandem repeat profiling

Objective To identify the passage stability of HEK293 cell which used for vaccine research by short tandem repeat (STR) profiling.Methods HEK293 cell line original from American type culture collection (ATCC)(USA) was passages and set as master seed bank (P40),working seed bank (P43) and manufacturing terminal cell (P65) according the requirements of China pharmacopoeia,and applied STR profiling step by step of cell amplification,DNA extraction,PCR amplification and STR profiling by 20 sites.Results Compared of STR profiling by 20 sites to 9 sites (ATCC) and 16 sites (NICPBP),the STR 20 sites covered all important indicates of 9 sites and 16 sites.Three different passages of HEK 293 P40,P43 and P65 were matched 100％ to ATCC standard cell line marked as 293EcRShh (Adv5),and were in accord with NICPBP test results and also had no exogenous cell pollution.Conclusions The passage stability of the HEK293 cell line is stabilize,has no exogenous cell pollution,and fits to the requirements of vaccine research. Key words: Tandem repeat;  HEK293;  Passage stability of cell